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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been noticed in lots of tumors, which leads to chromosome instability, and so may perhaps add to tumorigenesis. This protein was observed to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a important purpose in promoting mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are explained. Two more splice variants were being claimed but in depth nucleotide sequence info will not be but available.
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Blocking peptides are peptides that bind particularly on the target antibody and block antibody binding. These peptide typically incorporates the epitope recognized because of the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the very best effects are...
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This guide outlines additional feasible optimization of cellular and immunoassay parameters to make certain the absolute best final results are...
Antibodies certain to the blocking peptide not bind for the epitope about the goal protein. By comparing the staining in the blocked antibody compared to the antibody alone, you can see which staining is unique.
Concentrate on Facts Cyclin E belongs to your hugely conserved cyclin family, whose customers are characterised by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which contribute to your temporal coordination of every mitotic occasion. Cyclin E forms a posh with and functions for a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates in the G1-S section boundary which is degraded as cells progress as a result of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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